Biotechnology of Plant-Microbe Interactions and Naturals Products: Polisaccharides and Oligosaccharide
Francisco Pérez Montaño (PI)
Assistant Professor
Department of Microbiology, Faculty of Biology, University of Seville (BIO169)
Miguel Ángel Rodríguez Carvajal (PI)
Associate Professor
Department of Organic Chemistry, Faculty of Chemistry, University of Seville (BIO135)
Other group members
Research lines
- Regulation and conditions to produce nodulation factors
- Structure of oligosaccharides and polysaccharides
- Structure of other bioactive molecules, including AHLs, phytohormones and flavonoids
- Improvement of symbiotic process by using purified molecules involved in nodulation
Representative publications
- Del Cerro P, Ayala-García P, Buzón P, Castells-Graells R, López-Baena FJ, Ollero FJ, Pérez-Montaño F (2020) OnfD, an AraC-type transcriptional regulator encoded by Rhizobium tropici CIAT 899 and involved in Nod factor synthesis and symbiosis. Appl Env Microbiol 86: 19 (doi: 10.1128/AEM.01297-20).
- del Cerro P, Megías M, López-Baena FJ, Gil-Serrano A, Pérez-Montaño F, Ollero FJ (2019) Osmotic stress activates nif and fix genes and induces the Rhizobium tropici CIAT 899 Nod factor production via NodD2 by up-regulation of the nodA2 operon and the nodA3 PLoS One 14: e0213298 (doi: 10.1371/journal.pone.0213298).
- del Cerro P, Ayala-García P, Jiménez-Guerrero I, López-Baena FJ, Vinardell JM, Megías M, Hungría M, Gil-Serrano AM, Pérez-Montaño F, Ollero FJ (2019) The non-flavonoid inducible nodA3 and the flavonoid regulated nodA1 genes of Rhizobium tropici CIAT 899 guarantee nod factor production and nodulation of different host legumes. Plant Soil 440: 185-200.
- Acosta-Jurado S, Rodríguez-Navarro DN, Kawaharada Y, Rodríguez-Carvajal MA, Gil-Serrano A, Soria-Díaz ME, Pérez-Montaño F, Fernández-Perea J, Niu Y, Alias-Villegas C, Jiménez-Guerrero I, Navarro-Gómez P, López-Baena FJ, Kelly S, Sandal N, Stougaard J, Ruiz-Sainz JE, Vinardell JM (2019) Sinorhizobium fredii HH103 nolR and nodD2 mutants gain capacity for infection thread invasion of Lotus japonicus Gifu and Lotus burttii. Environ Microbiol 21: 1718-1739.
- Sánchez-Matamoros RC, Gil-Serrano AM, Espuny MR, Ollero FJ, Megías M, Rodríguez-Carvajal MA. (2018). Structure of surface polysaccharides from Aeromonas AMG272, a plant-growth promoting rhizobacterium isolated from rice rhizosphere. Carbohydr Res 462: 1-6.
- Jiménez-Guerrero I, Acosta-Jurado S, Del Cerro P, Navarro-Gómez P, López-Baena FJ, Ollero FJ, Vinardell JM, Pérez-Montaño F (2018) Transcriptomic studies of the effect of nod gene-inducing molecules in rhizobia: different weapons, one purpose. Genes 9: 1 (doi: 10.3390/genes9010001).
- Toro N, Villadas PJ, Molina-Sanchez MD, Navarro-Gomez P, Vinardell JM, Cuesta-Berrio L, Rodríguez-Carvajal MA (2017) The underlying process of early ecological and genetic differentiation in a facultatitve mutualistic Sinorhizobium meliloti Sci Rep 7: 675 (doi: 10.1038/s41598-017-00730-7).
- Acosta-Jurado S, Navarro-Gómez P, Crespo-Rivas JC, Medina C, Murdoch PS, Cuesta-Berrio L, Rodríguez-Carvajal MA, Ruiz-Sainz JE, Vinardell JM (2017) The Sinorhizobium (Esinfer) fredii HH103 rkp-2 region is involved in the biosynthesis of lipopolysaccharide and exopolysaccharide but not in K-antigen polysaccharide production. Plant Soil 417: 415-431.
- del Cerro P, Pérez-Montaño F, Gil-Serrano A, López Baena FJ, Megías M, Hungria M, Ollero FJ (2017) The Rhizobium tropici CIAT 899 NodD2 protein regulates the production of Nod factors under salt stress in a flavonoid-independent manner. Sci Rep 7: 46712 (doi: 10.1038/srep46712).
- del Cerro P, Rolla-Santos AAP, Valderrama-Fernández R, Gil-Serrano AM, Bellogín RA, Gomes DF, Pérez-Montaño F, Megías M, Hungria M, Ollero FJ (2016) NrcR, a new transcriptional regulator of Rhizobium tropici CIAT 899 involved in the legume root-nodule symbiosis. PLoS One 11: e0154029 (doi: 10.371/journal.pone.0154029).
- Pérez-Montaño F, del Cerro P, Jiménez-Guerrero I, López-Baena FJ, Cubo T, Hungria M, Megías M, Ollero FJ (2016) RNA-seq analysis of the Rhizobium tropici CIAT 899 transcriptome shows similarities in the activation patterns of symbiotic genes in the presence of apigenin and salt. BMC Genomics 17: 198 (doi: 10.1186/s12864-016-2543-3).
- Acosta-Jurado S, Alias-Villegas C, Navarro-Gómez P, Zehner S, Murdoch PS, Rodríguez-Carvajal MA, Soto J, Ollero FJ, Ruiz-Sainz JE, Göttfert M, Vinardell JM (2016) The Sinorhizobium fredii HH103 MucR1 global regulator is connected with the nod regulon and is required for efficient symbiosis with Lotus burttii and Glycine max Williams. Mol Plant Microbe Interact 29: 700-712.
- Pérez-Mendoza D, Rodríguez-Carvajal MA, Romero-Jiménez L, de Araujo Faira G, Loret J, Gallegos MT, Sanjuán J (2015) Novel mixed-linkage beta-glucan activated by c-di-GMP in Sinorhizobium meliloti. Proc Natl Acad Sci USA 112: E757-E765.
- del Cerro P, Rolla-Santos AAP, Gomes DF, Marks B, Pérez-Montaño F, Rodríguez-Carvajal MA, Gil-Serrano A, Megías M, Ollero FJ, Hungría M (2015) Regulatory nodD1 and nodD2 genes of Rhizobium tropici strain CIAT899 and their role in early steps of molecular signaling and host nodulation. BMC Genomics 16: 251 (doi: 10.1186/s12864-015-1458-8).
- del Cerro P, Rolla-Santos AAP, Gomes DF, Marks BB, Espuny MR, Rodríguez-Carvajal MA, Soria-Díaz ME, Nakatani AS, Hungria M, Ollero FJ, Megías M (2015) Opening the “black box” of nodD3, nodD4 and nodD5 genes of Rhizobium tropici strain CIAT 899. BMC Genomics 16: 864 (doi: 10.1186/s12864-105-2033-z).
Grants
- Rodríguez-Navarro D (IP), Pérez-Montaño F, Camacho M, Ollero FJ, Perea F (2020-2023) Legumes in biodiversity-based farming systems in Mediterranean basin (LEGU-MED). Partnership for Research and Innovation in the Mediterranean Area-Ministerio de Ciencia e Innovación. PCI2020-112151.
- Ollero FJ (IP), Gil-Serrano A (co-IP), Bellogín RA, Cubo MT, Espuny MR, Merchán F, Rodríguez-Carvajal MA, Tejero-Mateo P (2016-2019) Regulación de la biosíntesis de factores de nodulación por Rhizobium tropici CIAT899: Implicaciones de su aplicación como inoculante molecular en leguminosas y Ministerio de Economía y Competitividad. AGL2016-77163-R.
- Ollero FJ (IP), Megías M (co-IP), Bellogín RA, Cubo MT, Espuny MR, Gil-Serrano A, Rodríguez-Carvajal MA, Soria-Díaz ME, Tejero-Mateo P (2013-2015) Estudio de la regulación de los genes de la biosíntesis de factores de nodulación producidos por Rhizobium tropici en presencia de estrés abiótico. Ministerio de Economía y Competitividad. AGL2012-38831.
- Ollero FJ (IP), Gil-Serrano A (2019-2021) Producción de metabolitos bacterianos mediante fermentación de rizobacterias para su aplicación en inoculantes moleculares al servicio de una agricultura más sostenible. Agrogenia Biotech, S.L. 3525/0745.
- Ollero FJ (IP), Gil-Serrano A, Cuesta-Berrio L (2017-2018) Estudio de incorporación de microorganismos a semillas de interés industrial y ensayos de validación de los productos generados con interés comercial. Semillas Fitó, S.A. 3086/0745.
Relevant methods
- Extraction, purification and structural determination of lipoquitooligosaccharides (LCOs), acyl-homoserine lactones (AHLs) and siderophores
- In vitro determination of biological activity of LCOs
- Purification and structural determination of surface polysaccharides (EPS, CG, KPS and LPS)
- Nodulation assays, nodulation kinetics and competitiveness tests
- Bacterial and plant gene expression studies using qPCR, RNA-seq and dual RNA-seq
- Determination of enzymatic activities in bacteria (chitinase, pectinase, cellulase, urease, solubilization of phosphate, siderophores…) and in plants (peroxidase, MDA, superoxide dismutase…)
Collaborations with other national and international research groups
- Saul Burdman (Universidad de Rehovot, Israel)
- Mariangela Hungria (EMBRAPA Soja, Brazil)
- Federico Martinelli (Università degli studi di Palermo, Italy)
- Myriam Champentier (John Innes Centre, UK)
- Javier Lloret (Universidad Autónoma de Madrid, Spain)
- Dulce Rodríquez-Navarro y Maria Camacho (IFAPA Las Torres y Tomejil, Spain)
