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Metal Homeostasis in the Plant-Microorganism Interaction

Manuel González-Guerrero (IP)

Professor
Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid-Instituto Nacional de Investigaciones Agrarias, Madrid

ORCID

0000-0001-7334-5286

EMAIL

manuel.gonzalez@upm.es

PHONE NUMBER

+ 34 910679190 Ext:79190

Other group members

Viviana P. Escudero Welsch

Postdoctoral

Cristina Navarro Gómez

Estudiante Predoctoral

Elena Rosa Núñez

Estudiante Predoctoral

Alejandro M. Armas

Postdoctoral

Qingnan Chu

Postdoctoral

Cuong Hoang van.

Postdoctoral

Aishee De.

Postdoctoral

Juan Andrés Collantes García

PhD

Mario Rodríguez Simón

Technician

Elisa de Ansorena Pablos

Technician

Iker Eritz Torrico Crosnier

Technician

Research lines

  • Transport of metals in legume nodules
  • Metal exchange in arbuscular mycorrhizae
  • Metal trafficking in biological nitrogen fixation
  • Transition metals and innate plant immunity

Representative publications

  • González-Guerrero M, Navarro-Gómez C, Rosa-Núñez E, Echávarri-Erasun C, Imperial J, Escudero V (2023). Forging a symbiosis: Transition metal delivery in symbiotic nitrogen fixation. New Phytol. 239: 2113-2125.
  • Mihelj P, Abreu I, Moreyra T, González-Guerrero M, Raimunda R (2023). Functional characterization of the Co2+tranporter AitP in Sinorhizobium meliloti: a new player in Fe2+ homeostasis. Appl. Env. Microbiol. 89: e0190122
  • Assunçao AGL, Cakmak I, Clemens S, González-Guerrero M, Nawrocki A, Thomine S (2022). Micronutrient homeostasis in plants for more sustainable agriculture and healthier human nutrition. J. Exp. Bot. 73: 1789-1799
  • Escudero V, Ferreira- Sánchez D, Abreu I, Sopeña-Torres S, Makarovsky-Saavedra N, Bernal M, Krämer U, Grolimund D, González-Guerrero M, Jordá L (2022). Arabidopsis thaliana Zn2+–efflux ATPases HMA2 and HMA4 are required for resistance to the necrotrophic fungus Plectosphaerella cucumerina BMM. J. Exp. Bot. 73: 339-350. 
  • Castro-Rodríguez R, Escudero V, Reguera M, Gil-Diez P, Quintana J, Prieto RI, Kumar RK, Brear E, Grillet L, Wen J, Mysore KS, Walker EL, Smith PMC, Imperial J, González-Guerrero M (2021). Medicago truncatula Yellow Stripe-Like7 encodes a peptide transporter required for symbiotic nitrogen fixation. Plant Cell Environ. 44: 1908-1920
  • Gavrin A, Loughlin PC, Brear E, Griffith OW, Bedon F, Grotemeyer MS, Escudero V, Reguera M, Qu Y, Mohd-Noor SN, Chen C, Orsorio MB, Rentsch D, González-Guerrero M, Day DA, Smith PMC (2021). Soybean Yellow Stripe-Like7 is a symbiosome membrane peptide essential for nitrogen fixation. Plant Physiol. 186: 581-598
  • Senovilla M, Abreu I, Escudero V, Cano C, Bago A, Imperial J, González-Guerrero M (2020) MtCOPT2 is a Cu+ transporter specifically expressed in Medicago truncatula mycorrhizal roots. Mycorrhiza 30: 781-788.
  • Castro-Rodríguez R, Abreu I, Reguera M, Novoa-Aponte L, Mijovilovich A, Escudero V, Jiménez-Pastor FJ, Abadía J, Wen J, Mysore KS, Álvarez-Fernández A, Küpper H, Imperial J, González-Guerrero M (2020) Medicago truncatula Yellow Stripe1-Like3 gene is involved in symbiotic nitrogen fixation. J Exp Bot (doi: 10.1093/jxb/eraa390).
  • Escudero V, Abreu I, Tejada-Jiménez M, Rosa-Núñez E, Quintana J, Prieto RI, Larue C, Wen J, Villanova J, Mysore KS, Argüello JM, Castillo-Michel H, Imperial J, González-Guerrero M (2020) Medicago truncatula Ferroportin2 mediates iron import into nodule symbiosomes. New Phytol 228: 194-209.
  • Escudero V, Abreu I, del Sastre E, Tejada-Jiménez M, Larue C, Novoa-Aponte L, Castillo-González J, Wen J, Mysore KS, Abadía J, Argüello JM, Castillo-Michel H, Álvarez-Fernández A, Imperial J, González-Guerrero M (2020) Nicotianamine synthase2 is required for symbiotic nitrogen fixation in Medicago truncatula Front Plant Sci 10: 1780.
  • Ibeas MA, Grant-Grant S, Coronas MF, Vargas JI, Navarro N, Abreu I, Castillo-Michel H, Ávalos-Cembrano N, Paez-Valencia J, Pérez F, González-Guerrero M, Roschzttardtz H (2019) The diverse iron distribution in Eudicotyledoneae seeds: from Arabidopsis to Quinoa. Front Plant Sci 9: 1985 (doi: 3389/fpls.2018.01985).
  • Gil-Díez P, Tejada-Jiménez M, León-Mediavilla J, Wen J, Mysore KS, Imperial J, González-Guerrero M (2019) MtMOT1.2 is responsible for molybdate supply to Medicago truncatula Plant Cell Environ 42: 310-320.
  • León-Mediavilla J, Senovilla M, Montiel J, Gil-Díez P, Saez A, Kryvoruchko IS, Reguera M, Udvardi MK, Imperial J, González-Guerrero M (2018) MtMTP2-facilitated zinc transport into intracelular compartments is essential for nodule development in Medicago truncatula. Front Plant Sci 9: 990 (doi: 10.3389/fpls.2018.00990).
  • Senovilla M, Castro-Rodríguez R, Abreu I, Escudero V, Kryvoruchko I, Udvardi MK, Imperial J, González-Guerrero M (2018) Medicago truncatula copper transporter1 (MtCOPT1) delivers copper for symbiotic nitrogen fixation. New Phytol 218: 696-709.
  • Kryvoruchko I, Routray P, Senjuti S, Torres-Jerez I, Tejada-Jiménez M, Finney LA, Nakashima J, Pislariu CI, Benedito VA, González-Guerrero M, Roberts DM, Udvardi MK (2018) An iron-activated citrate transporter, MtMATE67, is required for symbiotic nitrogen fixation. Plant Physiol 176: 2315-2329.
  • Tejada-Jiménez M, Gil-Díez P, León-Mediavilla J, Wen J, Mysore KS, Imperial J, González-Guerrero M (2017) Medicago truncatula Molybdate Transporter type1 (MtMOT1.3) is a plasma membrane molybdenum transporter required for nitrogenase activity in root nodules under molybdenum deficiency. New Phytol 216: 1223-1235.
  • Abreu I, Sáez A, Castro-Rodríguez R, Escudero V, Rodríguez-Haas B, Senovilla M, Larue C, Grolimund D, Tejada-Jiménez M, Imperial J, González-Guerrero M (2017) Medicago truncatula Zinc-Iron Permease6 provided zinc to rhizobia-infected nodule cells. Plant Cell Environ 40: 2706-2719.
  • González-Guerrero M, Escudero V, Saéz A, Tejada-Jiménez M (2016) Transition metal transport in plants and associated endosymbionts: Arbuscular mycorrhizal fungi and rhizobia. Front Plant Sci 29:1088 (doi: 10.3389/fpls.2016.01088).
  • Tejada-Jiménez M, Castro-Rodríguez R, Kryvoruchko IS, Lucas MM, Udvardi M, Imperial J, González-Guerrero M (2015) Medicago truncatula Natural Resistance Associated Macrophage Protein1 is required for iron uptake by rhizobia-infected nodule cells. Plant Physiol 168: 258-275.
  • Zielazinski EL*, González-Guerrero M*, Subramanian P, Stemmler TL, Argüello JM, Rosenzweig AC (2013) Sinorhizobium meliloti Nia is a P1B-5-ATPase expressed in the nodule during plant symbiosis and is involved in Ni and Fe transport. Metallomics 5: 1614-1623.
  • Rodríguez-Haas B, Finney L, Vogt S, González-Melendi P, Imperial J, González-Guerrero M (2013) Iron distribution through the developmental stages of Medicago truncatula Metallomics 5: 1247-1253.

Grants

  • González-Guerrero M (IP) (2022-2024). Cytosolic iron trafficking for biological nitrogen fixation. Ministerio de Ciencia e Innovación PID2021-12460OB-100.
  • Jordá L, González-Guerrero M (IPs). Death by metals: Unveiling the molecular bases of zinc-mediated immunity. Ministerio de Ciencia en Innovación. TED2021-131769B-100
  • Rubio L (PI), González-Guerrero M (2020-2024) BNF-Cereals Phase III. Iron and Mo delivery to pathways to nitrogenase. Bill & Melinda Gates Foundation.
  • González-Guerrero M (PI) (2019-2021) Role of plant Cu+-chaperones in intracellular copper trafficking in symbiotic nitrogen fixation. Ministry of Science, Innovation and Universities. PGC2018-095996-B.
  • González-Guerrero M (PI) (2016-2018) Diverting metals to Medicago truncatula Ministry of Economy and Competitiveness. AGL-2015-65866-P.
  • González-Guerrero M (PI) (2014-2019) Metal transport in the tripartite symbiosis arbuscular mycorrhizal fungi-legume-rhizobia. European Research Council Starting Grant.

Relevant methods

  • Confocal and electronic microscopy
  • Visualisation of transition metals in plant tissues
  • Purification of membrane proteins
  • Metal transport tests on yeasts

Collaborations with other national and international groups

    • Luis M. Rubio (Centro de Biotecnología y Genómica de Plantas, UPM-INIA)
    • Lucía Jordá (Centro de Biotecnología y Genómica de Plantas, UPM-INIA)
    • Juan Imperial (Instituto de Ciencias Agrarias, CSIC)
    • Alberto Bago (Estación Experimental del Zaidín, CSIC)
    • Javier Abadía, Ana Álvarez (Estación Experimental de Aula Dei, CSIC)
    • José M. Argüello (Worcester Polytechnic Institute, EEUU)
    • Luis Vidali (Worcester Polytechnic Institute, EEUU)
    • Dennis Dean (Virginia Tech, EEUU)
    • Lydia Finney (Argonne National Lab, EEUU)
    • Michael K. Udvardi (Noble Research Institute, EEUU)
    • Kirankumar S. Mysore (Noble Research Institute, EEUU)
    • Amy Rosenzweig (Northwestern University, EEUU)
    • Daniel Raimunda (Instituto Ferreyra, Argentina)
    • Leonardo Curatti (Instituto Investigaciones en Biodiversidad y Biotecnología, Argentina)
    • Hannetz Roschzttardtz (Pontificia Universidad Católica de Chile, Chile)
    • Camille Larue (ECOLAB, Francia)
    • Hiram Castillo (European Synchrotron Radiation Facility, Francia)
    • Ute Krämer (Ruhr Universität Bochum, Alemania)
    • Daniel Grolimund (Swiss Light Source, Suiza)
    • Hendrik Küpper (Czech Academy of Sciences, Rep. Checa)
    • Louis Grillet (Academia Sinica, Taiwan)
    • Penelope Smith (La Trobe University, Australia)
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