Beneficial Plant-Microbe Interactions
José Javier Pueyo Dabad (IP)
Professor CSIC
TELÉFONO
+34 91 745 25 00 Ext: 950102
Other group members
Research lines
- Study of Legume-Rhizobium and Ectomycorrhizal symbioses
- Analysis of microbial diversity in agricultural and forestry systems
- Evaluation of the response of plants and micro-organisms to abiotic stresses and global change
- Phytoremediation and soil restoration through symbiotic associations
Representative publications
- Bhat A, Sharma R, Desigan K, (…), Pueyo JJ, Paape T (2024) Horizontal gene transfer of the Mer operon is associated with large effects on the transcriptome and increased tolerance to mercury in nitrogen-fixing bacteria. BMC Microbiology 24, 247
- Fedorova EE, Pueyo JJ (2024) Microbial Colonization of the Host Plant: Cellular and Molecular Mechanisms of Symbiosis. International Journal of Molecular Sciences 25, 639. https://doi.org/10.3390/ijms25010639
- Yahaghi Z, Shirvani M, Nourbakhsh F, Pueyo JJ (2024) Isolation, identification, and application of zinc‑solubilizing bacteria exhibiting beneficial traits to promote plant growth and metal uptake. International Journal of Environmental Science and Technology. http://doi.org/10.1007/s13762-024-06064-y
- Cordero I, Pueyo JJ, Rincón A (2024) Bio-fertilisation with native plant growth promoting rhizobacteria increases the tolerance of the neotropical legume tree Caesalpinia spinosa to water deficit. Forest Ecology and Management 558, 121786.
- Acuña B, Rincón A, Valladares F, Cordero I, Bravo-Oviedo A (2024) Natural forest colonisation as a management strategy to restore soil functioning of abandoned agricultural lands. Journal of Applied Ecology. http://doi.org/10.1111/1365-2664.14780
- Barou V, Rincón A, Parladé J (2024) Modelling environmental drivers of Tuber melanosporum extraradical mycelium in productive holm oak plantations and forests. Forest Ecology and Management 563, 121988. https://doi.org/10.1016/j.foreco.2024.121988
- Prieto-Rubio J, Garrido JL, Alcántara JM, Azcón-Aguilar C, Rincón A; López-García A (2024) Ectomycorrhizal fungal network complexity determines soil enzymatic functionality. Soil 10(1): 425-439. https://doi.org/10.5194/egusphere-2024-119
- Garrido JL, Azcón C, López-García A, Ozuna C, Perea A, Prieto J, Rincón A, Alcántara J (2023) The structure and ecological function of the interactions between plants and arbuscular mycorrhizal fungi through multilayer networks. Functional Ecology 37: 2217-2230. http://doi.org/10.1111/1365-2435.14378
- Barou V, Rincón A, Calvet C, Camprubí A, Parladé J (2023) Aromatic plants and their associated arbuscular mycorrhizal fungi outcompete Tuber melanosporum in compatibility assays with truffle-oaks. Biology-Basel 12(4): 628-640. https://doi.org/10.3390/biology12040628
- Prieto-Rubio J, Perea A, Garrido JL, Alcántara JM, Azcón-Aguilar C, López-García A, Rincón A (2023) Plant traits and phylogeny predict soil carbon and nutrient cycling in Mediterranean mixed forests. Ecosystems 26: 1047-1060. https://doi.org/10.1007/s10021-022-00815-z
- Bahena-Osorio Y, Franco-Hernández MO, Pueyo JJ, Vásquez-Murrieta MS (2023) Development of a quality index to evaluate the impact of abiotic stress in saline soils in the geothermal zone of Los Negritos, Michoacán, Mexico. Agronomy 13, 1659. https://doi.org/10.3390/agronomy13061650
- Msaddak A, Quiñones MA, Mars M, Pueyo JJ (2023) The beneficial effects of inoculation with selected nodule-associated PGPR on white lupin are comparable to those of inoculation with symbiotic rhizobia. Plants 12, 4109. https://doi.org/10.3390/plants12244109
- Pacheco-Insausti MC, Ponce IT, Quiñones MA, Pedranzani HE, Pueyo JJ (2023) Effects of inoculation with stress-tolerant rhizobia on the response of alfalfa (Medicago sativa L.) to combined salinity and cadmium stress. Plants 12, 3972. https://doi.org/10.3390/plants12233972
- Msaddak A, Mars M, Quiñones MA, Lucas MM, Pueyo JJ (2023) Lupin, a unique legume that is nodulated by multiple microsymbionts: the role of horizontal gene transfer. International Journal of Molecular Sciences 24, 6496. https://doi.org/10.3390/ijms24076496
- Prieto-Rubio J, López-García A, Pérez-Izquierdo L, Garrido JL, Alcántara JM, Azcón-Aguilar C, Rincón A (2022) Scale dependency of ectomycorrhizal fungal community assembly processes in Mediterranean mixed forests. Mycorrhiza 32 (3-4): 315-325. https://doi.org/10.1007/s00572-022-01083-4
- Paape T, Heiniger B, Santo Domingo M, Clear M, Lucas MM, Pueyo JJ (2022) Genome-wide association study reveals complex genetic architecture of cadmium and mercury accumulation and tolerance traits in Medicago truncatula. Frontiers in Plant Science 12, 806949. https://doi.org/10.3389/fpls.2021.806949
- Quiñones MA, Lucas MM, Pueyo JJ (2022) Adaptive mechanisms make lupin a choice crop for acidic soils affected by aluminium toxicity. Frontiers in Plant Science 12, 810692. https://doi.org/10.3389/fpls.2021.810692
- Garcia de la Torre VS, Coba de la Peña T, Lucas MM, Pueyo JJ (2022) Transgenic Medicago truncatula plants that accumulate proline display enhanced tolerance to cadmium stress. Frontiers in Plant Science 13, 829069. https://doi.org/10.3389/fpls.2022.829069
- Trifonova NA, Kamyshinsky R, Coba de la Peña T, Koroleva MI, Kulikova O, Lara-Dampier V, Pashkovskiy P, Presniakov M, Pueyo JJ, Lucas MM, Fedorova EE (2022) Sodium accumulation in infected cells and ion transporters mistargeting in nodules of Medicago truncatula: Two ugly items that hinder coping with salt stress effects. Journal of Molecular Sciences 23, 10618. https://doi.org/10.3390/ijms231810618
- Álvarez-Rivera G, Sanz A, Cifuentes A, Ibáñez E, Paape T, Lucas M, Pueyo JJ (2022) Flavonoid accumulation varies in Medicago truncatula in response to mercury stress. Frontiers in Plant Science 13, 933209. https://doi.org/10.3389/fpls.2022.933209
- Cordero I, Jiménez MD, Delgado JA, Balaguer L, Pueyo JJ, Rincón A (2021) Local adaptation optimizes photoprotection strategies in a Neotropical legume tree under drought stress. Tree Physiology 41 (9): 1641-1657. https://doi.org/10.1093/treephys/tpab034
- Pueyo JJ, Quiñones MA, Coba de la Peña T, Fedorova EE, Lucas MM (2021) Nitrogen and phosphorus interplay in lupin root nodules and cluster roots. Frontiers in Plant Science 12, 644218. https://doi.org/10.3389/fpls.2021.644218
- Quiñones MA, Fajardo S, Fernández-Pascual M, Lucas MM, Pueyo JJ (2021) Nodulated white lupin plants growing in contaminated soils accumulate unusually high mercury concentrations in their nodules, roots and especially cluster roots. Horticulturae 7, 302. https://doi.org/10.3390/horticulturae7090302
- Fedorova EE, Coba de la Peña T, Lara-Dampier V, Trifonova NA, Kulikova O, Pueyo JJ, Lucas MM (2021) Potassium content diminishes in infected cells of Medicago truncatula nodules due to the mislocation of channels MtAKT1 and MtSKOR/GORK. Journal of Experimental Botany, 72, 1336-1348. https://doi.org/ 10.1093/jxb/eraa508
- Arregui G, Hipólito P, Pallol B, Lara-Dampier V, García-Rodríguez D, Varela HP, Tavakoli Zaniani P, Balomenos D, Paape T, Coba de la Peña T, Lucas MM, Pueyo JJ (2021) Mercury-tolerant Ensifer medicae strains display high mercuric reductase activity and a protective effect on nitrogen fixation in Medicago truncatula nodules under mercury stress. Frontiers in Plant Science 11, 560768. https://doi.org/10.3389/fpls.2020.560768
- García de la Torre VS, Coba de la Peña T, Pueyo JJ, Lucas MM (2021) Cadmium-tolerant and -sensitive cultivars identified by screening of Medicago truncatula germplasm display contrasting responses to cadmium stress. Frontiers in Plant Science 12, 595001. https://doi.org/10.3389/fpls.2021.595001
- Msaddak A, Rey L, Imperial J, Palacios JM, Mars M, Pueyo JJ (2021) Phylogenetic analyses of rhizobia isolated from nodules of Lupinus angustifolius in northern tunisia reveal Devosia sp. as a new microsymbiont of lupin species. Agronomy 11, 1510. https://doi.org/10.3390/agronomy11081510
- Fedorova EE, Coba de la Peña T, Lara-Dampier V, Kulikova O, Pueyo JJ, Lucas MM (2020) Potassium content diminishes in infected cells of Medicago truncatula nodules due to the mislocation of channels MtAKT1 and MtSKOR/GORK. J Exp Bot (doi: 10.1093/jxb/ eraa508).
- Pérez-Izquierdo L, Zabal-Aguirre M, Verdú M, Buée M, Rincón A (2020) Ectomycorrhizal fungal diversity decreases in Mediterranean pine forests adapted to recurrent fires. Mol Ecol 29: 2463-2476.
- Pérez-Izquierdo L, Zabal-Aguirre M, González-Martínez SC, Buée M, Verdú M, Rincón A, Goberna M (2019) Plant intraspecific variation modulates nutrient cycling through its below-ground rhizospheric microbiome. J Ecol 107: 1594-1605.
- Hmaeid N, Wali M, Metoui O, Pueyo JJ, Ghanaya T, Abdelly C (2019) Efficient rhizobacteria promote growth and alleviate NaCl-induced stress in the plant species Sulla carnosa. Appl Soil Ecol 133: 104-113.
Grants
- Rincón A (Coord., IP, SP1), Peguero-Pina JJ (IP, SP2), Bonet JA (IP, SP3) (2023-2027) TUBERLINKS. Evaluación de la biodiversidad y las relaciones suelo-planta en sistemas productores de trufa para el óptimo manejo y gestión de sus producciones. PID2022-1364780B-C31. Ministerio de Ciencia e Innovación, MICINN, Agencia Estatal de Investigación, AEI.
- Rincón A (IP), Pueyo JJ (CoIP) (2022-2024) MIDIVERSOIL. Evaluación de estrategias basadas en la biodiversidad microbiana para la restauración y remediación del suelo. TED2021-132350B-I00. Ministerio de Ciencia e Innovación, MICINN, Plan Recuperación, Transformación y Resiliencia. Agencia Estatal de Investigación, AEI.
- Pueyo JJ (IP), Lucas MM (CoIP) (2022-2026) LUPIMETAL. White lupin and its associated bacteria as a heavy metal phytoremediation tool with environmental and agronomic added value. PID2021-125371OB-I00. Ministerio de Ciencia e Innovación, MICINN, Agencia Estatal de Investigación, AEI.
- Pueyo JJ (IP), Mars M (CoIP) (2022-2023) Applications of the symbiotic association legume-rhizobium in the phytoremediation of heavy metal polluted soils. COOPB20610. CSIC, Programa de Cooperación Científica para el Desarrollo i-COOP
- Rincón A (IP) (2019-2022) Diseño e implementación de sistemas multiproductivos sostenibles en truficultura y análisis de los servicios ecosistémicos asociados. Ministerio de Ciencia, Innovación y Universidades. RTI2018-093907-B-C22. Coordinado con IRTA, Parladé X (Coordinador).
- Pueyo JJ (IP), Lucas MM (IP) (2018-2021) Respuesta de la simbiosis rizobio-leguminosa a metales pesados: de análisis ómicos a la fitorremediación de suelos contaminados. Ministerio de Ciencia e Innovación. AGL2017-88381-R.
- Pueyo JJ (2017-2019) Utilización de variedades de alfalfa en la fitorremediación de suelos salinos y contaminados por metales pesados. CSIC, MHE-200034.
Relevant methods
- Molecular characterization of microorganisms
- Metagenomics of microbial communities
- Immunochemical techniques
- Optical, fluorescence, confocal laser and transmission electron microscopy
- Real-time quantitative PCR
- Electrophoresis of nucleic acids, proteins and lipopolysaccharides
- PAGE-native, determination of enzyme activities
- Gas chromatography, measurement of acetylene reduction activity
- Analysis of plant growth promoting capacities of bacterial isolates
- Formulation of ectomycorrhizal fungal inocula
- Determination of soil and mycorrhizal enzyme activities
- Ecophysiology of plants
Collaborations with other national and international research groups
- Cristina Aponte, INIA-CSIC, Madrid
- Xavier Parladé Izquierdo (IRTA, Cabrils, Barcelona)
- Ana María de Miguel Velasco (Universidad de Navarra)
- Agustín Probanza (Universidad San Pablo CEU, Madrid)
- Concepción Azcón (Estación Experimental del Zaidín, CSIC, Granada)
- Julio Alcántara (Universidad de Jaén)
- Fernando Valladares (Museo Nacional de Ciencias Naturales, CSIC, Madrid)
- Jorge Curiel Yuste (Basque Centre for Climate Change, Bilbao)
- Miguel Verdú del Campo (Centro de Investigaciones sobre Desertificación, CSIC, Valencia)
- Marta Goberna Estellés (INIA, Madrid)
- Elena Ibáñez Ezequiel (ICTAN, CSIC, Madrid)
- Manuel González-Guerrero (CBGP, Universidad Politécnica de Madrid)
- Jose Enrique Ruiz-Sainz y Jose María Vinardell (Universidad de Sevilla)
- Teodoro Coba de la Peña (CEAZA, La Serena, Chile)
- Timothy Paape (Brookhaven National Laboratory, Upton, NY, USA)
- Hilda Pedranzani (Universidad Nacional de San Luis, San Luis, Argentina)
- Elena Fedorova (KA Timiryazev Institute of Plant Physiology, Russian Academy of Science, Moscú, Russia)
- Soledad Vásquez-Murrieta (Instituto Politécnico Nacional, Ciudad de México, Mexico)
- Marc Buée y Laurent Saint André (INRA, Nancy, France)
